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Article
Comparison of high performance liquid Chromatography, Radioimmunoassay and electrochemiluminescence Immunoassay for quantification of serum 25 hydroxy vitamin D
Clinical Biochemistry
  • Lena Jafri, Aga Khan University
  • Aysha Habib Khan, Aga Khan University
  • Anwar Ali Siddiqui, Aga Khan University
  • Shamim Mushtaq, Aga Khan University
  • Romaina Iqbal, Aga Khan University
  • Farooq Ghani, Aga Khan University
  • Imran Siddiqui, Aga Khan University
Publication Date
7-1-2011
Document Type
Article
Abstract
Objective: To compare the performance of radioimmunoassay (RIA) with high-performance liquid chromatography (HPLC) and electrochemiluminescence (ECLIA) for the quantification of vitamin D (25OHD). Methods: HPLC method for the determination of 25OHD in human biological samples was developed and compared in terms of accuracy and precision with a commercially available RIA assay. Performance of RIA assay with ECLIA technology for 25OHD analysis was further compared. Results: Median 25OHD levels with HPLC vs. RIA were 50.1 nmol/L (IQ = 17.7–199.4 nmol/L) and 51.1 nmol/L (IQ = 12.5–187.2 nmol/L) respectively, whereas median 25OHD concentration with RIA vs. ECLIA was 32.4 nmol/L (9.98–199.7 nmol/L) and 29.9 nmol/L (4.9–214.6 nmol/L), respectively. Comparison data for HPLC vs. RIA showed RIA = − 1.13 + 1.01 (HPLC) (RMSE = 11.2 nmol/L) and for RIA vs. ECLIA revealed, ECLIA = 3.21 + 0.9 (RIA) (RMSE9.6 nmol/L). Conclusion: Acceptable correlation was observed among HPLC and RIA and also with RIA and ECLIA in quantification of 25OHD.
Citation Information
Lena Jafri, Aysha Habib Khan, Anwar Ali Siddiqui, Shamim Mushtaq, et al.. "Comparison of high performance liquid Chromatography, Radioimmunoassay and electrochemiluminescence Immunoassay for quantification of serum 25 hydroxy vitamin D" Clinical Biochemistry Vol. 44 Iss. 10-11 (2011) p. 864 - 868
Available at: http://works.bepress.com/aysha_habib/18/