Norepinephrine Induces Systolic Failure and Inhibits Antiapoptotic Genes in a Polymicrobial Septic Rat ModelLife Sciences
AbstractAIMS: We examined the effect of norepinephrine (NE) infusion on left ventricular function and apoptotic genes during progression of polymicrobial sepsis. METHODS: Male Sprague-Dawley rats (350-400 g) were made septic by intraperitoneal (i.p.) administration of 200mg/kg cecal inoculum. Sham animals received 5% dextrose water, i.p. Echocardiography was performed at baseline, 3 days and 7 days post-sepsis/sham. NE (0.6 μgkg(-1)h(-1)) was infused for 2h, before the end of day 3 of echocardiography. At the end of day 7, rats were euthanized and heart tissues harvested for isolation of total RNA. PCR was performed using RT(2) profiler™ PCR array PARN-012 (Rat apoptosis array; SuperArray, MD) using RT(2) Real-Time™ SYBR Green PCR master mix PA-012. KEY FINDINGS: NE-infusion resulted in a significant decrease in the left ventricular ejection fraction (EF) (62.56±2.07 from the baseline 71.11±3.23, p SIGNIFICANCE: The data suggest that upregulation of a series of pro-apoptotic molecules could be responsible for systolic and diastolic dysfunction during 3 and 7 days post sepsis.
Citation InformationMani Chopra, Padmalaya Das, Honey Golden, David E. Dostal, et al.. "Norepinephrine Induces Systolic Failure and Inhibits Antiapoptotic Genes in a Polymicrobial Septic Rat Model" Life Sciences Vol. 87 Iss. 23-26 (2010) p. 672 - 678
Available at: http://works.bepress.com/avadhesh_sharma/2/