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Article
Evaluation of spontaneous generation of allelic variation in soybean in response to sexual hybridization and stress
Canadian Journal of Plant Science
  • Katherine Espinosa, Iowa State University
  • Jessica Boelter, University of Wisconsin - Stevens Point
  • Susan Lolle, University of Waterloo
  • Marianne Hopkins, University of Waterloo
  • Susana Goggi, Iowa State University
  • Reid G. Palmer, Iowa State University
  • Devinder Sandhu, University of Wisconsin-Stevens Point
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
3-1-2015
DOI
10.1139/CJPS-2014-324
Abstract

Intra-cultivar variation reported in pure lines of soybean has been hypothesized to result from genetic mechanisms contributing to de novo genetic variation. In this study we have detected allele switching by following segregation pattern of Aconitase-4 isozyme in sexual crosses and pure lines. In sexual crosses, one F2 plant showed switch at the Aconitase- 4 (Aco4) locus from the expected heterozygous genotype Aco4-ac to Aco4-ab. In the pure lines grown in a honeycomb planting design and treated with an accelerated aging test, multiple cases of allele switching were detected at the Aco4 locus. Both single and double switches were detected that were stable and heritable. These findings indicate that the generation of endogenous variation continues in pure lines as a result of intrinsic genetic mechanisms. With a long-term goal of understanding the genetic nature of the changes, we genetically mapped the Aco4 gene to a 3.3 cM region on Chromosome 11. The corresponding physical region is ~293 kb with 39 predicated genes. Of these, Glyma.11g080600 is of particular interest, as it shows 93% and 88% identity to Medicago truncatula and Arabidopsis aconitase genes, respectively. Further characterization of the soybean Aco4 gene may shed light on genetic mechanisms responsible for allele switching.

Comments

This is a manuscript of an article published as Espinosa, Katherine, Jessica Boelter, Susan Lolle, Marianne Hopkins, Susana Goggi, Reid G. Palmer, and Devinder Sandhu. "Evaluation of spontaneous generation of allelic variation in soybean in response to sexual hybridization and stress." Canadian Journal of Plant Science 95, no. 2 (2015): 405-415. doi: 10.1139/CJPS-2014-324. Posted with permission.

Copyright Owner
The Authors
Language
en
File Format
application/pdf
Citation Information
Katherine Espinosa, Jessica Boelter, Susan Lolle, Marianne Hopkins, et al.. "Evaluation of spontaneous generation of allelic variation in soybean in response to sexual hybridization and stress" Canadian Journal of Plant Science Vol. 95 Iss. 2 (2015) p. 405 - 415
Available at: http://works.bepress.com/asusana-goggi/7/