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Article
Evaluation of Antioxidant and Free Radical Scavenging Activities of Oxystelma esculentum in Various In Vitro Models
Journal of Complementary and Integrative Medicine (2008)
  • Durairaj Ashokkumar
  • Upal Kanti Mazumder
  • Malaya Gupta
  • GP Senthilkumar
  • Vaiyapuri Thamil Selvan
Abstract

Objective - The present study was aimed to evaluate the antioxidant and free radical scavenging properties of Oxystelma esculentum (MEOE).

Methods- The total antioxidant, reducing power, DPPH radical, superoxide anion radical, nitric oxide radical, hydroxyl radical, hydrogen peroxide scavenging and total phenol content in MEOE was carried out.

Results - The total antioxidant activity of MEOE increases with increasing concentration. The reducing capability and free radical scavenging activity in DPPH also increased in a dose dependent manner. The MEOE was found to scavenge the superoxide generated by PMS/NADH/NBT system. Moreover, MEOE was found to inhibit the nitric oxide radical generated from sodium nitropruside. MEOE was also found to inhibit the hydroxyl radical generated by Fe3+/ascorbate/EDTA/H2O2 system. The MEOE scavenges the hydrogen peroxide in a dose dependent manner. The amount of total phenolic compounds was also determined.

Conclusion - The results suggest that the methanol extract of Oxystelma esculentum proved its strong antioxidant activity and can be used as a natural antioxidant.

Keywords
  • lipid peroxidation,
  • DPPH radical,
  • superoxide scavenging,
  • total phenolic content,
  • Oxystelma esculentum
Publication Date
October 16, 2008
Citation Information
Durairaj Ashokkumar, Upal Kanti Mazumder, Malaya Gupta, GP Senthilkumar, et al.. "Evaluation of Antioxidant and Free Radical Scavenging Activities of Oxystelma esculentum in Various In Vitro Models" Journal of Complementary and Integrative Medicine Vol. 5 Iss. 1 (2008)
Available at: http://works.bepress.com/ashokkumardurairaj/1/