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Selected Works of Asheesh Singh
Presentation
Identification of chromosomal regions associated with gluten index for marker-assisted selection in durum wheat
10th International Gluten workshop, 7 - 9 September 2009. (2009)
  • F. Clarke, Agriculture and Agri-Food Canada
  • C. Pozniak, University of Saskatchewan
  • J. Clarke, University of Saskatchewan
  • R. Knox, Agriculture and Agri-Food Canada
  • N. Ames, Agriculture and Agri-Food Canada
  • A. K. Singh, Agriculture and Agri-Food Canada
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Abstract
Gluten strength is an important end-use quality factor in durum wheat (Triticum turgidum L. ssp. durum
(Desf.) Husn.). Gluten index has become widely-used for selection in durum breeding programs because
of its simplicity and independence from protein concentration. Our unpublished results indicate that gluten
index is complexly inherited, so identification of suitable DNA markers for marker-assisted selection is a
priority. Doubled haploid populations derived from the F1 of three crosses of strong and weak gluten
parents were grown in replicated field trials at two or three locations for two years. Gluten index was
determined on whole meal samples from each plot, and the populations were genotyped with SSR and/or
DArT (Diversity Arrays Technology) markers. One population was also assessed on a plot basis using
SDS-sedimentation on whole meal samples and the alveograph on purified semolina, to compare the
gluten index method with other strength measures. Quantitative trait loci (QTL) for gluten index were
detected in one or more of the three populations on chromosomes 1B, 2B, 4A, 5B, and 6B, confirming the
complex inheritance of this trait. The 1B QTL was also associated with phenotypic variation for SDSsedimentation
volume and alveograph strength (W). The gluten index QTL on 6B was not associated with
variation for any of the alveograph measures, but was associated with SDS-sedimentation volume. The
DArT markers listed here have identified several chromosome regions that will be further examined with
additional DNA markers (microsatellite and single nucleotide polymorphic (SNP) markers).
Disciplines
Publication Date
2009
Location
Clermont-Ferrand, France
Comments
This proceeding was published as Clarke FR, CJ Pozniak, JM Clarke, RE Knox, N Ames, AK Singh (2009). Identification of chromosomal regions associated with gluten index for marker-assisted selection in durum wheat. In G. Branlard (ed) proc. 10th International Gluten Workshop. Sept 7 – 9, 2009. Clermont Ferrand, France; INRA, Paris.  pp 231-234. Posted with permission.
Citation Information
F. Clarke, C. Pozniak, J. Clarke, R. Knox, et al.. "Identification of chromosomal regions associated with gluten index for marker-assisted selection in durum wheat" 10th International Gluten workshop, 7 - 9 September 2009. (2009)
Available at: http://works.bepress.com/asheesh-singh/9/