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A Polyadenylation Factor Subunit Implicated in Regulating Oxidative Signaling in Arabidopsis thaliana
PLoS One
  • Jingxian Zhang, University of Kentucky
  • Bala Subrahmanyam Addepalli, University of Kentucky
  • Kil-Young Yun, University of Massachusetts
  • Arthur G. Hunt, University of Kentucky
  • Ruqiang Xu, Miami University
  • Suryadevara Rao, University of Kentucky
  • Qingshun Q. Li, Miami University
  • Deane L. Falcone, University of Massachusetts
Abstract
BACKGROUND: Plants respond to many unfavorable environmental conditions via signaling mediated by altered levels of various reactive oxygen species (ROS). To gain additional insight into oxidative signaling responses, Arabidopsis mutants that exhibited tolerance to oxidative stress were isolated. We describe herein the isolation and characterization of one such mutant, oxt6. METHODOLOGY/PRINCIPAL FINDINGS: The oxt6 mutation is due to the disruption of a complex gene (At1g30460) that encodes the Arabidopsis ortholog of the 30-kD subunit of the cleavage and polyadenylation specificity factor (CPSF30) as well as a larger, related 65-kD protein. Expression of mRNAs encoding Arabidopsis CPSF30 alone was able to restore wild-type growth and stress susceptibility to the oxt6 mutant. Transcriptional profiling and single gene expression studies show elevated constitutive expression of a subset of genes that encode proteins containing thioredoxin- and glutaredoxin-related domains in the oxt6 mutant, suggesting that stress can be ameliorated by these gene classes. Bulk poly(A) tail length was not seemingly affected in the oxt6 mutant, but poly(A) site selection was different, indicating a subtle effect on polyadenylation in the mutant. CONCLUSIONS/SIGNIFICANCE: These results implicate the Arabidopsis CPSF30 protein in the posttranscriptional control of the responses of plants to stress, and in particular to the expression of a set of genes that suffices to confer tolerance to oxidative stress.
Document Type
Article
Publication Date
6-11-2008
Disciplines
Notes/Citation Information

Published in PLoS One, v. 3, no. 6, p. 2410.

© 2008 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Digital Object Identifier (DOI)
http://dx.doi.org/10.1371/journal.pone.0002410
Citation Information
Jingxian Zhang, Bala Subrahmanyam Addepalli, Kil-Young Yun, Arthur G. Hunt, et al.. "A Polyadenylation Factor Subunit Implicated in Regulating Oxidative Signaling in Arabidopsis thaliana" PLoS One Vol. 3 Iss. 6 (2008) p. 2410 - 2410
Available at: http://works.bepress.com/arthur_hunt/3/