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Mapping molecular datasets back to the brain regions they are extracted from: Remembering the native countries of hypothalamic expatriates and refugees
In press, Advances in Neurobiology (this is the preprint) (2018)
  • Arshad M. Khan
  • Alice H Grant, University of Texas at El Paso
  • Anais Martinez, University of Texas at El Paso
  • Gully APC Burns, University of Southern California
  • Brendan S Thatcher, VA Puget Sound Health Care System
  • Vishwanath T Anekonda, VA Puget Sound Health Care System
  • Benjamin W Thompson, VA Puget Sound Health Care System
  • Zachary S Roberts, VA Puget Sound Health Care System
  • Daniel H Moralejo, University of Washington School of Medicine
  • James E Blevins, University of Washington School of Medicine
Abstract
This article, which includes novel unpublished data along with commentary and analysis,
focuses on approaches to link transcriptomic, proteomic, and peptidomic datasets mined from
brain tissue to the original locations within the brain that they are derived from using digital atlas
mapping techniques. We use, as an example, the transcriptomic, proteomic and peptidomic
analyses conducted in the mammalian hypothalamus. Following a brief historical overview, we
highlight studies that have mined biochemical and molecular information from the hypothalamus
and then lay out a strategy for how these data can be linked spatially to the mapped locations in a
canonical brain atlas where the data come from, thereby allowing researchers to integrate these
data with other datasets across multiple scales. A key methodology that enables atlas-based
mapping of extracted datasets – laser capture microdissection – is discussed in detail, with a view
of how this technology is a bridge between systems biology and systems neuroscience.
Keywords
  • hypothalamus,
  • proteomics,
  • peptidomics,
  • transcriptomics,
  • laser capture,
  • brain mapping
Publication Date
June 11, 2018
Citation Information
Arshad M. Khan, Alice H Grant, Anais Martinez, Gully APC Burns, et al.. "Mapping molecular datasets back to the brain regions they are extracted from: Remembering the native countries of hypothalamic expatriates and refugees" In press, Advances in Neurobiology (this is the preprint) (2018)
Available at: http://works.bepress.com/arshad_m_khan/27/