A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris ecology, geographic distribution, and public health risk.
- Balamuthia; DNA; ecology; molecular; PCR; protozoa; ribosomal; sequence
Available at: http://works.bepress.com/arinefadzlun_ahmad/4/