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Article
Development of a Nested PCR for Environmental Detection of the Pathogenic Free-Living Amoeba Balamuthia mandrillaris
Journal of Eukaryotic Microbiology (2011)
  • Arine Fadzlun Ahmad, University of Malaya
Abstract

A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris ecology, geographic distribution, and public health risk.

Keywords
  • Balamuthia; DNA; ecology; molecular; PCR; protozoa; ribosomal; sequence
Disciplines
Publication Date
May, 2011
Citation Information
Arine Fadzlun Ahmad. "Development of a Nested PCR for Environmental Detection of the Pathogenic Free-Living Amoeba Balamuthia mandrillaris" Journal of Eukaryotic Microbiology Vol. 58 Iss. 3 (2011)
Available at: http://works.bepress.com/arinefadzlun_ahmad/4/