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Article
Determination of Alpha-i Antitrypsin Genetic Deficiency in Duodenal Ulcer by Polymerase Chain Reaction
Journal Of Pakistan Medical Association
  • A Shahid, Jinnah Postgraduate Medical Centre, Karachi.
  • H Qureshi, Jinnah Postgraduate Medical Centre, Karachi
  • S J Zuberi, Jinnah Postgraduate Medical Centre, Karachi
  • Anwar Ali Siddiqui, Aga khan University
  • T Sultana, Aga khan University
  • M A Waqar, Aga khan University
Publication Date
1-1-2002
Document Type
Article
Abstract
Objective: To confirm alpha-I-AT deficiency status in duodenal ulcer using a combination of PCR and restricted enzyme digestion. Methods: Fifty patients with endoscopically proven duodenal ulcer and hundred controls with no signs of the disease were included. Alpha-i-AT phenotypes were confirmed by polymerase chain reaction followed by restriction enzyme digestion. Results:Alpha-I-AT concentration in duodenal ulcer patients showed a mean value of 2.12 ± 0.11g/1 (range: 0.52-3.95 g/1, p Conclusion: Alpha-1 AT deficiency was found in 10% of duodenal ulcer patients. DNA analysis more accurately resolved the phenotypes as S and Z mutations (JPMA 52:545; 2002).
Citation Information
A Shahid, H Qureshi, S J Zuberi, Anwar Ali Siddiqui, et al.. "Determination of Alpha-i Antitrypsin Genetic Deficiency in Duodenal Ulcer by Polymerase Chain Reaction" Journal Of Pakistan Medical Association Vol. 52 Iss. 12 (2002) p. 545 - 548
Available at: http://works.bepress.com/anwar_siddiqui/18/