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Presentation
Pulse-field-gel-electrophoresis development for Salmonella species using PaeR7 I enzyme
International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
  • Curtis Jerald Thompson, Iowa State University
  • James D. McKean, Iowa State University
  • Annette M. O'Connor, Iowa State University
Publication Date
2007
Abstract
Pulse field gel electrophoresis (PFGE) currently has been preformed on 22 Salmonella spp. isolates using a new method that distinguishes between different species of Salmonella and gives reproducible results using Pae R7 1 enzyme. This PFGE data, when using the Pae R7 1 enzyme, was compared to the Xba 1 restriction enzyme that is used by CDC (Center for Disease Control) for Salmonella spp isolate comparisons. PFGE results were analyzed using cluster analysis and results were comparable between Pae R7 1 and Xba 1 enzymes for distinguishing differences.
Book Title
7th International Symposium on the Epidemiology & Control of Foodborne Pathogens in Pork
Pages
496-497
Language
en
File Format
application/pdf
Citation Information
Curtis Jerald Thompson, James D. McKean and Annette M. O'Connor. "Pulse-field-gel-electrophoresis development for Salmonella species using PaeR7 I enzyme" (2007)
Available at: http://works.bepress.com/annette_oconnor/27/