Recent research on psychopathy has begun to explore two dimensions that possibly underlie psychopathy–one related more to emotional and interpersonal traits, and another related more to antisocial behaviors. A community sample of adults was assessed for psychopathy using Hare's (1991) Psychopathy Checklist-Revised (PCL-R). Eyeblinks elicited by startle probes were recorded while participants viewed pictures of emotionally-laden stimuli. Consistent with previous research, participants scoring high on PCL-R Factor 2 (“antisocial”) showed no affective modification of startle if they also scored high on PCL-R Factor 1 (“emotional detachment”). When the factor scores were analyzed together as continuous variables in a regression analysis, however, affective modification of startle was negatively related to Factor 1 but positively related to Factor 2. The results thus provide further support for a two-factor model of psychopathy.