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Isolation of Diverse Members of the Aquificales from Geothermal Springs in Tengchong, China
Frontiers in Microbiology
  • Brian P. Hedlund, University of Nevada, Las Vegas
  • Anna-Louise Reysenbach, Portland State University
  • Liuquin Huang, China University of Geosciences
  • John C. Ong, University of Nevada, Las Vegas
  • Zizhang Liu, China University of Geosciences
  • Jeremy A. Dodsworth, University of Nevada, Las Vegas
  • Reham Ahmed, University of Nevada, Las Vegas
  • Amanda J. Williams, University of Nevada, Las Vegas,
  • Brandon R. Briggs, Miami University - Oxford
  • Yitai Liu, Portland State University
  • Weiguo Hou, China University of Geosciences
  • Hailiang Dong, China University of Geosciences
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  • Geothermal resources -- China,
  • Thermophilic microorganisms -- China -- Tengchong,
  • Thermophilic bacteria -- Isolation,
  • Thermophilic bacteria -- Phylogeny

The order Aquificales (phylum Aquificae) consists of thermophilic and hyperthermophilic bacteria that are prominent in many geothermal systems, including those in Tengchong, Yunnan Province, China. However, Aquificales have not previously been isolated from Tengchong. We isolated five strains of Aquificales from diverse springs (temperature 45.2–83.3°C and pH 2.6–9.1) in the Rehai Geothermal Field from sites in which Aquificales were abundant. Phylogenetic analysis showed that four of the strains belong to the genera Hydrogenobacter, Hydrogenobaculum, andSulfurihydrogenibium, including strains distant enough to likely justify new species ofHydrogenobacter and Hydrogenobaculum. The additional strain may represent a new genus in theHydrogenothermaceae. All strains were capable of aerobic respiration under microaerophilic conditions; however, they had variable capacity for chemolithotrophic oxidation of hydrogen and sulfur compounds and nitrate reduction.


Copyright 2015 Hedlund, Reysenbach, Huang, Ong, Liu, Dodsworth , Ahmed, Williams, Briggs, Liu, Houand Dong.This is an open-access article distributed under the terms of the Creative Commons Attribution License (CCBY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hedlund, B. P., Reysenbach, A. L., Huang, L., Ong, J. C., Liu, Z., Dodsworth, J. A., ... & Dong, H. (2015). Isolation of diverse members of the Aquificales from geothermal springs in Tengchong, China. Frontiers in microbiology, 6.