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Isolation of diverse members of the Aquificales from geothermal springs in Tengchong, China
Frontiers in Microbiology
  • Brian P Hedlund, University of Nevada, Las Vegas
  • Anna-Louise Reysenbach
  • Liuquin Huang
  • John Ong
  • Zizhang Liu
  • Jeremy Dodsworth
  • Reham Ahmed
  • Amanda Williams
  • Brandon Briggs
  • Yitai Liu
  • Weiguo Huo
  • Hailiang Dong
Document Type
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The order Aquificales (phylum Aquificae) consists of thermophilic and hyperthermophilic bacteria that are prominent in many geothermal systems, including those in Tengchong, Yunnan Province, China. However, Aquificales have not previously been isolated from Tengchong. We isolated five strains of Aquificales from diverse springs (temperature 45.2–83.3°C and pH 2.6–9.1) in the Rehai Geothermal Field from sites in which Aquificales were abundant. Phylogenetic analysis showed that four of the strains belong to the genera Hydrogenobacter, Hydrogenobaculum, and Sulfurihydrogenibium, including strains distant enough to likely justify new species of Hydrogenobacter and Hydrogenobaculum. The additional strain may represent a new genus in the Hydrogenothermaceae. All strains were capable of aerobic respiration under microaerophilic conditions; however, they had variable capacity for chemolithotrophic oxidation of hydrogen and sulfur compounds and nitrate reduction.

  • Aquifcales,
  • Hydrogenobacter,
  • Hydrogenobaculum,
  • Sulfrihydrogenibium,
  • Hot springs,
  • Hydrogen oxidation,
  • Sulfide oxidation,
  • Thiosulfate oxidation
Citation Information
Brian P Hedlund, Anna-Louise Reysenbach, Liuquin Huang, John Ong, et al.. "Isolation of diverse members of the Aquificales from geothermal springs in Tengchong, China" Frontiers in Microbiology Vol. 6 (2015) p. 157
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