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Article
Genomics-Informed Isolation and Characterization of a Symbiotic Nanoarchaeota System from a Terrestrial Geothermal Environment
Nature Communications
  • Louie Wurch, Oak Ridge National Laboratory
  • Richard J. Giannone, Oak Ridge National Laboratory
  • Bernard S. Belisle, Oak Ridge National Laboratory
  • Carolyn Swift, Oak Ridge National Laboratory
  • Sagar Utturkar, Oak Ridge National Laboratory
  • Robert L. Hettich, Oak Ridge National Laboratory
  • Anna-Louise Reysenbach, Portland State University
  • Mircea Podar, University of Tennessee - Knoxville
Document Type
Article
Publication Date
7-5-2016
Subjects
  • Archaebacteria -- Genetic aspects,
  • Molecular biology,
  • Thermophilic microorganism,
  • Archaebacteria -- Evolution
Abstract

Biological features can be inferred, based on genomic data, for many microbial lineages that remain uncultured. However, cultivation is important for characterizing an organism’s physiology and testing its genome-encoded potential. Here we use single-cell genomics to infer cultivation conditions for the isolation of an ectosymbiotic Nanoarchaeota (‘Nanopusillus acidilobi’) and its host (Acidilobus, a crenarchaeote) from a terrestrial geothermal environment. The cells of ‘Nanopusillus’ are among the smallest known cellular organisms (100–300 nm). They appear to have a complete genetic information processing machinery, but lack almost all primary biosynthetic functions as well as respiration and ATP synthesis. Genomic and proteomic comparison with its distant relative, the marine Nanoarchaeum equitans illustrate an ancient, common evolutionary history of adaptation of the Nanoarchaeota to ectosymbiosis, so far unique among the Archaea.

Description

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. Copyright 2016 The Author(s). Supplementary Information

DOI
10.1038/ncomms12115
Persistent Identifier
http://archives.pdx.edu/ds/psu/18029
Citation Information
Wurch, L. et al. Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment. Nat. Commun. 7:12115 doi: 10.1038/ncomms12115 (2016).