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Article
Isolation of a Ubiquitous Obligate Thermoacidophilic Archaeon From Deep-Sea Hydrothermal Vents
Nature
  • Anna-Louise Reysenbach, Portland State University
  • Yitai Liu, Portland State University
  • Amy B. Banta, Portland State University
  • Terry J. Beveridge, University of Guelph
  • Julie D. Kirshtein, US Geological Survey
  • Stefan Schouten, Royal Netherlands Institute for Sea Research
  • Margaret K. Tivey, Woods Hole Oceanographic Institution
  • Karen L. Von Damm, University of New Hampshire
  • Mary A. Voytek, US Geological Survey
Document Type
Post-Print
Publication Date
5-1-2006
Subjects
  • Hydrothermal vents -- Microbiology,
  • Submarine geology,
  • Deep-sea ecology
Disciplines
Abstract
Deep-sea hydrothermal vents are important in global biogeochemical cycles, providing biological oases at the sea floor that are supported by the thermal and chemical flux from the Earth's interior. As hot, acidic and reduced hydrothermal fluids mix with cold, alkaline and oxygenated sea water, minerals precipitate to form porous sulphide–sulphate deposits. These structures provide microhabitats for a diversity of prokaryotes that exploit the geochemical and physical gradients in this dynamic ecosystem. It has been proposed that fluid pH in the actively venting sulphide structures is generally low (pH < 4.5), yet no extreme thermoacidophile has been isolated from vent deposits. Culture-independent surveys based on ribosomal RNA genes from deep-sea hydrothermal deposits have identified a widespread euryarchaeotal lineage, DHVE2 (deep-sea hydrothermal vent euryarchaeotic 2) Despite the ubiquity and apparent deep-sea endemism of DHVE2, cultivation of this group has been unsuccessful and thus its metabolism remains a mystery. Here we report the isolation and cultivation of a member of the DHVE2 group, which is an obligate thermoacidophilic sulphur- or iron-reducing heterotroph capable of growing from pH 3.3 to 5.8 and between 55 and 75 °C. In addition, we demonstrate that this isolate constitutes up to 15% of the archaeal population, providing evidence that thermoacidophiles may be key players in the sulphur and iron cycling at deep-sea vents.
Description

This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in Nature 442 (2006): 444-447.

Copyright 2006 Nature Publishing Group

DOI
10.1038/nature04921
Persistent Identifier
http://archives.pdx.edu/ds/psu/10665
Citation Information
Anna-Louise Reysenbach, Yitai Liu, Amy B. Banta, Terry J. Beveridge, et al.. "Isolation of a Ubiquitous Obligate Thermoacidophilic Archaeon From Deep-Sea Hydrothermal Vents" Nature (2006)
Available at: http://works.bepress.com/anna-louise_reysenbach/1/