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Presentation
Analysis of N-linked Oligosaccharides from Xenopus laevis Vitelline Envelope Glycoprotein ZPA by Mass Spectrometry
239th American Chemical Society (ACS) National Meeting
  • Liang Zhao, University of the Pacific
  • Fan Xiang, Shimadzu Biotech Corporation
  • Jerry L. Hedrick, University of California, Davis
  • Andreas H. Franz, University of the Pacific
Document Type
Conference Presentation
Department
Chemistry
Organization
American Chemical Society (ACS)
Location
San Francisco, CA
Conference Dates
March 21-25, 2010
Date of Presentation
3-23-2010
Disciplines
Abstract
Glycoprotein ZPA, is the least abundant component of the four vitelline envelop glycoproteins (ZPA, ZPB, ZPC and ZPX) of Xenopus laevis egg. Oligosaccharides from these types of glycoproteins are believed to play key roles in fertilization process, which may include protection of eggs against pathogens, initiation of sperm capacitation or the acrosome reaction, species-specific interaction of gametes, and prevention of polyspermy. Knowledge of the glycosylation of ZPA is rudimentary and no structural details for the glycans are known. Here we report progress towards the structure elucidation of N-linked glycans from ZPA. After SDS-PAGE separation, ZPA was firstly in-gel digested followed by enrichment through lectin affinity and/or liquid chromatography. Glycans were released from the glycopeptides through PNGaseF. Both glycopeptides and glycans were characterized by mass spectrometry.
Citation Information
Liang Zhao, Fan Xiang, Jerry L. Hedrick and Andreas H. Franz. "Analysis of N-linked Oligosaccharides from Xenopus laevis Vitelline Envelope Glycoprotein ZPA by Mass Spectrometry" 239th American Chemical Society (ACS) National Meeting (2010)
Available at: http://works.bepress.com/andreas-franz/95/