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Article
High-Throughput One-Bead-One-Compound Approach to Peptide-Encoded Combinatorial Libraries: MALDI-MS Analysis of Single TentaGelTM Beads
Journal of Combinatorial Chemistry
  • Andreas H. Franz, University of California, Davis
  • Ruiwu Liu, University of California, Davis
  • Aimin Song, University of California, Davis
  • Kit S. Lam, University of California, Davis
  • Carlito B. Lebrilla, University of California, Davis
Document Type
Article
Department
Chemistry
DOI
10.1021/cc020083a
Publication Date
1-18-2003
Disciplines
Abstract
The identification of pharmacologically promising compounds (lead compounds) from combinatorial libraries is frequently limited by the throughput of the analytical technique employed. Fourier transform mass spectrometry (FTMS) offers high sensitivity, mass accuracy (m/Δm > 500 000), and sequencing capabilities. A rapid and efficient method for high-throughput analysis of single beads from peptide-encoded combinatorial libraries with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is presented. Encoding peptides on single beads are identified and structurally characterized by MALDI time-of-flight (TOF) and ultrahigh-resolution MALDI Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. A strategy of on-probe sample preparation is developed to minimize handling of the beads.
Citation Information
Andreas H. Franz, Ruiwu Liu, Aimin Song, Kit S. Lam, et al.. "High-Throughput One-Bead-One-Compound Approach to Peptide-Encoded Combinatorial Libraries: MALDI-MS Analysis of Single TentaGelTM Beads" Journal of Combinatorial Chemistry Vol. 5 Iss. 2 (2003) p. 125 - 137 ISSN: 1520-4766
Available at: http://works.bepress.com/andreas-franz/131/