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Presentation
Chromatographic Separation of Oligosaccharide Mixtures Labeled with a Multifunctional Tag
Annual Conference of the Society for Glycobiology
  • Joanne Hsu, University of the Pacific
  • Soo Jin Chang, University of the Pacific
  • Andreas H. Franz, University of the Pacific
Document Type
Poster
Department
Chemistry
Organization
Society for Glycobiology
Location
Honolulu, HI
Conference Dates
November 17-20, 2004
Date of Presentation
11-17-2004
Disciplines
Abstract
New multifunctional tags for labeling carbohydrates by reductive amination were synthesized. The tags combine UV-activity, bioaffinity, and the possibility for quaternization. The structural identity of the tags was confirmed by NMR spectroscopy, by MALDI-TOF, and by ESI mass spectrometry. Several mixtures of monosaccharides and oligosaccharides labeled with the new multifunctional tag were separated by HPLC on porous graphitized carbon (PGC) and C18 media with acetonitrile/water/TFA gradients. The efficiency of separation was verified by off-line MALDI-TOF mass spectrometry. The linear oligosaccharide maltotriose and the human milk sugars LNFP 2 and LNDFH 2 were labeled as standards with and without deuterium coding. H/D mixtures of known compositions were analyzed for relative abundance by isotope patterns in MALDI-TOF mass spectrometry. Good agreement between predicted and observed values was observed.
Citation Information
Joanne Hsu, Soo Jin Chang and Andreas H. Franz. "Chromatographic Separation of Oligosaccharide Mixtures Labeled with a Multifunctional Tag" Annual Conference of the Society for Glycobiology (2004)
Available at: http://works.bepress.com/andreas-franz/105/