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Article
CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS
Journal of Applied Oral Science
  • Carla Renata Sipert, University of São Paulo
  • Ana Carolina Morandini, University of São Paulo
  • Karin Cristina da Silva Modena, University of São Paulo
  • Thiago José Dionísio, University of São Paulo
  • Maria Aparecida Andrade Moreira Machado, University of São Paulo
  • Sandra Helena Penha Oliveira, Universidade Estadual Paulista
  • Ana Paula Campanelli, University of São Paulo
  • Carlos Ferreira Santos, University of São Paulo
ORCiD
Ana C. Morandini: 0000-0003-4749-571X
Document Type
Article
DOI
10.1590/1678-7757201300004
Publication Date
3-1-2013
Disciplines
Abstract
OBJECTIVE: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS). MATERIAL AND METHODS: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0-10 µg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA. Comparisons among samples were performed using One-way ANOVA for MTT assay and Two-way ANOVA for ELISA results. RESULTS: Cell viability was not affected by the antigen after 24 h of stimulation. PgLPS induced the production of CCL3 by dental pulp fibroblasts at similar levels for both permanent and deciduous pulp fibroblasts. Production of CXCL12, however, was significantly higher for PDPF than DDPF at 1 and 6 h. PgLPS, in turn, downregulated the production of CXCL12 by PDPF but not by DDPF. CONCLUSION: These data suggest that dental pulp fibroblasts from permanent and deciduous teeth may present a differential behavior under PgLPS stimulation.
Creative Commons License
Creative Commons Attribution 4.0
Citation Information
Carla Renata Sipert, Ana Carolina Morandini, Karin Cristina da Silva Modena, Thiago José Dionísio, et al.. "CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS" Journal of Applied Oral Science Vol. 21 Iss. 2 (2013) p. 99 - 105 ISSN: 1678-7765
Available at: http://works.bepress.com/ana-morandini/6/