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Article
Trypanosoma cruzi invades host cells through the activation of endothelin and bradykinin receptors: a converging pathway leading to chagasic vasculopathy
British Journal of Pharmacology
  • Daniele Andrade, Universidade Federal do Rio de Janeiro
  • Rafaela Serra, Universidade Federal do Rio de Janeiro
  • Erik Svensjö, Universidade Federal do Rio de Janeiro
  • Ana Paula C. Lima, Universidade Federal do Rio de Janeiro
  • Erivan Schnaider Ramos-Junior, Universidade Federal do Rio de Janeiro
  • Fabio S. Fortes, Centro Universitário Estadual da Zona Oeste
  • Ana Carolina Morandini, Universidade de São Paulo
  • Verônica Morandi, Universidade do Estado do Rio de Janeiro
  • Maria de N Soeiro, Fundação Oswaldo Cruz
  • Herbert B. Tanowitz, Albert Einstein College of Medicine
  • Julio Scharfstein, Universidade Federal do Rio de Janeiro
ORCiD
Ana C. Morandini: 0000-0003-4749-571X
Document Type
Article
DOI
10.1111/j.1476-5381.2011.01609.x
Publication Date
3-1-2012
Disciplines
Abstract

BACKGROUND AND PURPOSE: Independent studies in experimental models of Trypanosoma cruzi appointed different roles for endothelin-1 (ET-1) and bradykinin (BK) in the immunopathogenesis of Chagas disease. Here, we addressed the hypothesis that pathogenic outcome is influenced by functional interplay between endothelin receptors (ET(A)R and ET(B)R) and bradykinin B(2) receptors (B(2)R).

EXPERIMENTAL APPROACH: Intravital microscopy was used to determine whether ETR/B(2)R drives the accumulation of rhodamine-labelled leucocytes in the hamster cheek pouch (HCP). Inflammatory oedema was measured in the infected BALB/c paw of mice. Parasite invasion was assessed in CHO over-expressing ETRs, mouse cardiomyocytes, endothelium (human umbilical vein endothelial cells) or smooth muscle cells (HSMCs), in the presence/absence of antagonists of B(2)R (HOE-140), ET(A)R (BQ-123) and ET(B)R (BQ-788), specific IgG antibodies to each GPCRs; cholesterol or calcium-depleting drugs. RNA interference (ET(A)R or ET(B)R genes) in parasite infectivity was investigated in HSMCs.

KEY RESULTS: BQ-123, BQ-788 and HOE-140 reduced leucocyte accumulation in HCP topically exposed to trypomastigotes and blocked inflammatory oedema in infected mice. Acting synergistically, ET(A)R and ET(B)R antagonists reduced parasite invasion of HSMCs to the same extent as HOE-140. Exogenous ET-1 potentiated T. cruzi uptake by HSMCs via ETRs/B(2)R, whereas RNA interference of ET(A)R and ET(B)R genes conversely reduced parasite internalization. ETRs/B(2)R-driven infection in HSMCs was reduced in HSMC pretreated with methyl-β-cyclodextrin, a cholesterol-depleting drug, or in thapsigargin- or verapamil-treated target cells.

CONCLUSIONS AND IMPLICATIONS: Our findings suggest that plasma leakage, a neutrophil-driven inflammatory response evoked by trypomastigotes via the kinin/endothelin pathways, may offer a window of opportunity for enhanced parasite invasion of cardiovascular cells.

Citation Information
Daniele Andrade, Rafaela Serra, Erik Svensjö, Ana Paula C. Lima, et al.. "Trypanosoma cruzi invades host cells through the activation of endothelin and bradykinin receptors: a converging pathway leading to chagasic vasculopathy" British Journal of Pharmacology Vol. 165 Iss. 5 (2012) p. 1333 - 1347 ISSN: 1476-5381
Available at: http://works.bepress.com/ana-morandini/19/