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Article
Evaluation of Systems for Collection of Porcine Zygotes for Dna Microinjection and Transfer
Theriogenology
  • B. L. Williams
  • Amy E.T. Sparks, University of Iowa
  • R. S. Canseco
  • J. W. Knight
  • J. L. Johnson
  • W. H. Velander
  • R. L. Page
  • W. N. Drohan
  • E. T. Kornegay
  • R. E. Pearson
  • T. D. Wilkins
  • F. C. Gwazdauskas
Document Type
Article
Peer Reviewed
1
Publication Date
9-1-1992
NLM Title Abbreviation
Theriogenology
DOI of Published Version
10.1016/0093-691X(92)90070-8
Abstract

Crossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were used: 1) sows were observed for natural estrous behavior; 1000 IU human chorionic gonadotrophin (hCG) was administered at the onset of estrus (NAT); 2) cyclic gilts were synchronized with 17.6 mg altrenogest (ALT)/day for 15 to 19 days followed by superovulation with 1500 IU pregnant mares serum gonadotropin (PMSG) and 500 IU hCG (LALT); 3) gilts between 11 and 16 days of the estrous cycle received 17.6 mg ALT for 5 to 9 days and PMSG and hCG were used to induce superovulation (SALT); and 4) precocious ovulation was induced in prepubertal gilts with PMSG and hCG (PRE). A total of 505 DNA microinjected embryos transferred into 17 recipients produced 7 litters and 50 piglets, of which 8 were transgenic. The NAT sows had less (P 0.05) in the number of corpora hemorrhagica or unovulated follicles, but SALT and PRE treatments had higher ovulation rates than LALT (24.7 +/- 2.9, 24.3 +/- 1.8 vs 11.6 +/- 2.7 ovulations; xBAR +/- SEM). The SALT and PRE treatments yielded 12.3 +/- 2.6 and 17.7 +/- 1.7 zygotes. Successful transgenesis was accomplished with SALT and PRE procedures for estrus synchronization and superovulation.

Published Article/Book Citation
Theriogenology, 38:3 (1992) pp.501-511.
Citation Information
B. L. Williams, Amy E.T. Sparks, R. S. Canseco, J. W. Knight, et al.. "Evaluation of Systems for Collection of Porcine Zygotes for Dna Microinjection and Transfer" Theriogenology Vol. 38 Iss. 3 (1992) p. 501 - 511 ISSN: 0093-691X
Available at: http://works.bepress.com/amy_sparks/68/