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Article
Effects of L-asparaginase on plasma amino acid profiles and tumor burden in cats with lymphoma
Journal of Veterinary Internal Medicine (2007)
  • Amy K LeBlanc, University of Tennessee - Knoxville
  • Sherry Cox, University of Tennessee - Knoxville
  • Claudia A Kirk, University of Tennessee - Knoxville
  • Shelley Newman, University of Tennessee - Knoxville
  • Joe Bartges, University of Tennessee - Knoxville
  • Alfred M Legendre, University of Tennessee - Knoxville
Abstract

BACKGROUND: L-Asparaginase (Elspar(a)), is an Escherichia coli-derived enzyme that depletes lymphoma cells of asparagine, inhibiting protein synthesis and resulting in cell death. The single agent response rate in cats with lymphoma and impact of L-asparaginase on plasma amino acid concentrations is unknown. HYPOTHESES: L-Asparaginase significantly reduces plasma asparagine concentrations and has demonstrable efficacy against untreated lymphoma in cats. ANIMALS: Thirteen cats with confirmed lymphoma (LSA) of any anatomic site were given 1 dose 400 IU/kg IM) of L-asparaginase for initial LSA treatment. METHODS: Plasma collected at 0, 2, and 7 days after L-asparaginase therapy was assayed for ammonia, asparagine, aspartic acid, glutamine, and glutamic acid concentrations. Cats were restaged 7 days later to assess tumor response. RESULTS: Eight cats had T-cell LSA, 4 cats had B-cell LSA, and 1 cat's immunophenotype was unknown. Two complete and 2 partial responses to L-asparaginase were seen. Four cats had stable disease, and 5 cats had progressive disease. Ammonia and aspartic acid concentrations were increased from baseline at 2 and 7 days posttreatment. Asparagine concentrations were decreased from baseline at 2 days but not 7 days posttreatment. Glutamic acid concentrations were increased at day 2 compared to day 7 posttreatment but not compared to baseline. Glutamine concentrations were unchanged. CONCLUSIONS AND CLINICAL IMPORTANCE: L-asparaginase significantly reduced asparagine concentrations within 2 days of treatment, but this effect was lost within 7 days. The apparent overall response rate of feline LSA to L-asparaginase in this study was 30%.

Keywords
  • Amino Acids,
  • Antineoplastic Agents,
  • Asparaginase,
  • Cat Diseases,
  • Cats,
  • Female,
  • Lymphoma,
  • Male
Disciplines
Publication Date
2007
Citation Information
Amy K LeBlanc, Sherry Cox, Claudia A Kirk, Shelley Newman, et al.. "Effects of L-asparaginase on plasma amino acid profiles and tumor burden in cats with lymphoma" Journal of Veterinary Internal Medicine Vol. 21 Iss. 4 (2007)
Available at: http://works.bepress.com/amy_leblanc/4/