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Article
Purification, Crystallization and Preliminary Crystallographic Analysis of the SH2 Domain of IL-2-inducible T-cell Kinase
Acta Crystallographica Section F
  • Raji E. Joseph, Iowa State University
  • Nathaniel D. Ginder, Iowa State University
  • Julie A. Hoy, Iowa State University
  • Jay C. Nix, United States Department of Energy
  • Richard B. Honzatko, Iowa State University
  • Amy H. Andreotti, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
2-1-2011
DOI
10.1107/S1744309110052346
Abstract
Proline is a unique amino acid owing to the relatively small energy difference between the cis and trans conformations of its peptide bond. The X–Pro imide bond readily undergoes cis–trans isomerization in the context of short peptides as well as some proteins. However, the direct detection of cis–trans proline isomerization in folded proteins is technically challenging. NMR spectroscopy is well suited to the direct detection of proline isomerization in folded proteins. It is less clear how well X-ray crystallography can reveal this conformational exchange event in folded proteins. Conformational heterogeneity owing to cis–trans proline isomerization in the Src homology 2 (SH2) domain of the IL-2-inducible T-cell kinase (ITK) has been extensively characterized by NMR. Using the ITK SH2 domain as a test system, an attempt was made to determine whether proline isomerization could be detected in a crystal structure of the ITK SH2 domain. As a first step towards this goal, the purification, crystallization and preliminary characterization of the ITK SH2 domain are described.
Comments

This article is from Acta Crystallographica Section F 67 (2011): 269, doi:10.1107/S1744309110052346.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Raji E. Joseph, Nathaniel D. Ginder, Julie A. Hoy, Jay C. Nix, et al.. "Purification, Crystallization and Preliminary Crystallographic Analysis of the SH2 Domain of IL-2-inducible T-cell Kinase" Acta Crystallographica Section F Vol. 67 Iss. 2 (2011) p. 269 - 273
Available at: http://works.bepress.com/amy_andreotti/5/