A Selective NMR Probe to Monitor the Conformational Transition from Inactive to Active KinaseACS Chemical Biology
Publication VersionPublished Version
AbstractKinases control many aspects of cellular signaling and are therefore therapeutic targets for numerous disease states. Monitoring the conformational changes that drive activation and inactivation of the catalytic kinase core is a challenging experimental problem due to the dynamic nature of these enzymes. We apply [13C] reductive methylation to chemically introduce NMR-active nuclei into unlabeled protein kinases. The results demonstrate that solution NMR spectroscopy can be used to monitor specific changes in the chemical environment of structurally important lysines in a [13C]-methylated kinase as it shifts from the inactive to active state. This approach provides a solution based method to complement X-ray crystallographic data and can be applied to nearly any kinase, regardless of size or method of production.
RightsThis is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Copyright OwnerAmerican Chemical Society
Citation InformationQian Xie, D. Bruce Fulton and Amy H. Andreotti. "A Selective NMR Probe to Monitor the Conformational Transition from Inactive to Active Kinase" ACS Chemical Biology Vol. 10 Iss. 1 (2014) p. 262 - 268
Available at: http://works.bepress.com/amy_andreotti/14/