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A Selective NMR Probe to Monitor the Conformational Transition from Inactive to Active Kinase
ACS Chemical Biology
  • Qian Xie, Iowa State University
  • D. Bruce Fulton, Iowa State University
  • Amy H. Andreotti, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
9-1-2014
DOI
10.1021/cb5004702
Abstract
Kinases control many aspects of cellular signaling and are therefore therapeutic targets for numerous disease states. Monitoring the conformational changes that drive activation and inactivation of the catalytic kinase core is a challenging experimental problem due to the dynamic nature of these enzymes. We apply [13C] reductive methylation to chemically introduce NMR-active nuclei into unlabeled protein kinases. The results demonstrate that solution NMR spectroscopy can be used to monitor specific changes in the chemical environment of structurally important lysines in a [13C]-methylated kinase as it shifts from the inactive to active state. This approach provides a solution based method to complement X-ray crystallographic data and can be applied to nearly any kinase, regardless of size or method of production.
Comments

This article is from ACS Chemical Biology 10 (2014): 262, doi:10.1021/cb5004702. Posted with permission.

Rights
This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Copyright Owner
American Chemical Society
Language
en
File Format
application/pdf
Citation Information
Qian Xie, D. Bruce Fulton and Amy H. Andreotti. "A Selective NMR Probe to Monitor the Conformational Transition from Inactive to Active Kinase" ACS Chemical Biology Vol. 10 Iss. 1 (2014) p. 262 - 268
Available at: http://works.bepress.com/amy_andreotti/14/