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Article
Lymantria dispar iflavirus 1 (LdIV1), a new model to study iflaviral persistence in lepidopterans
Journal of General Virology
  • Jimena Carrillo-Tripp, Iowa State University
  • Elizabeth N. Krueger, Iowa State University
  • Robert L. Harrison, United States Department of Agriculture, Agricultural Research Service
  • Amy L. Toth, Iowa State University
  • W. Allen Miller, Iowa State University
  • Bryony C. Bonning, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-2014
DOI
10.1099/vir.0.067710-0
Abstract
The cell line IPLB-LD-652Y, derived from the gypsy moth (Lymantria dispar L.), is routinely used to study interactions between viruses and insect hosts. Here we report the full genome sequence and biological characteristics of a small RNA virus, designated Lymantria dispar iflavirus 1 (LdIV1), that was discovered to persistently infect IPLB-LD-652Y. LdIV1 belongs to the genus Iflavirus. LdIV1 formed icosahedral particles of approx. 30 nm in diameter and contained a 10 044 nt polyadenylated, positive-sense RNA genome encoding a predicted polyprotein of 2980 aa. LdIV1 was induced by a viral suppressor of RNA silencing, suggesting that acute infection is restricted by RNA interference (RNAi). We detected LdIV1 in all tested tissues of gypsy-moth larvae and adults, but the virus was absent from other L. dispar-derived cell lines. We confirmed LdIV1 infectivity in two of these cell lines (IPLB-LD-652 and IPLB-LdFB). Our results provide a novel system to explore persistent infections in lepidopterans and a new model for the study of iflaviruses, a rapidly expanding group of viruses, many of which covertly infect their hosts.
Comments

This is an article from Journal of General Virology 95 (2014): 2285, doi:10.1099/vir.0.067710-0. Posted with permission.

Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Citation Information
Jimena Carrillo-Tripp, Elizabeth N. Krueger, Robert L. Harrison, Amy L. Toth, et al.. "Lymantria dispar iflavirus 1 (LdIV1), a new model to study iflaviral persistence in lepidopterans" Journal of General Virology Vol. 95 (2014) p. 2285 - 2296
Available at: http://works.bepress.com/amy-toth/6/