The objective of this teaching note is to develop a laboratory exercise, which allows students to get a hands-on experience of a molecular biology technique to analyze gene expression. The short duration of the biology laboratory for an undergraduate curriculum is the biggest challenge with the development of new labs. An important part of cell biology or molecular biology undergraduate curriculum is to study gene expression. There are many labs to study gene expression in qualitative manner. The commonly used reporter gene expression studies are primarily qualitative. However, there is no hands-on experience exercise to quantitatively determine gene expression. Therefore, it is necessary to design a laboratory exercise that enables the students to carry out cell or molecular biological assays in the desired time. Here we report a laboratory where we can introduce students to gene expression using the real time Quantitative Polymerase Chain Reaction (RT-qPCR) by comparative CT method to analyze expression of genes in Drosophila tissues.