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Article
Lobe and Serrate are Required for Cell Survival during Early Eye Development in Drosophila
Development
  • Amit Singh, University of Dayton
  • Xiao Shi, Baylor College of Medicine
  • Kwang-Wook Choi, Baylor College of Medicine
Document Type
Article
Publication Date
11-1-2006
Abstract
Organogenesis involves an initial surge of cell proliferation, leading to differentiation. This is followed by cell death in order to remove extra cells. During early development, there is little or no cell death. However, there is a lack of information concerning the genes required for survival during the early cell-proliferation phase. Here, we show that Lobe (L) and the Notch (N) ligand Serrate (Ser), which are both involved in ventral eye growth, are required for cell survival in the early eye disc. We observed that the loss-of-ventral-eye phenotype in L or Ser mutants is due to the induction of cell death and the upregulation of secreted Wingless (Wg). This loss-of-ventral-eye phenotype can be rescued by (i) increasing the levels of cell death inhibitors, (ii) reducing the levels of Hid-Reaper-Grim complex, or (iii) reducing canonical Wg signaling components. Blocking Jun-N-terminal kinase (JNK) signaling, which can induce caspase-independent cell death, significantly rescued ventral eye loss in L or Ser mutants. However, blocking both caspase-dependent cell death and JNK signaling together showed stronger rescues of the L- or Ser-mutant eye at a 1.5-fold higher frequency. This suggests that L or Ser loss-of-function triggers both caspase-dependent and -independent cell death. Our studies thus identify a mechanism responsible for cell survival in the early eye.
Inclusive pages
4771-4781
ISBN/ISSN
0950-1991
Document Version
Published Version
Comments

This document is provided for download in compliance with the publisher's policy on self-archiving. Permission documentation is on file.

Publisher
Company of Biologists
Peer Reviewed
Yes
Citation Information
Amit Singh, Xiao Shi and Kwang-Wook Choi. "Lobe and Serrate are Required for Cell Survival during Early Eye Development in Drosophila" Development Vol. 133 Iss. 23 (2006)
Available at: http://works.bepress.com/amit_singh/47/