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Article
Cell Type-Specific Responses to Wingless, Hedgehog and Decapentaplegic Are Essential for Patterning Early Eye-Antenna Disc in Drosophila
PLOS One
  • Jong-Hoon Won, Korea Advanced Institute of Science and Technology
  • Orkhon Tsogtbartarr, Korea Advanced Institute of Science and Technology
  • Wonseok Son, Korea Advanced Institute of Science and Technology
  • Amit Singh, University of Dayton
  • Kwang-Wook Choi, Baylor College of Medicine
  • Kyung-Ok Cho, Korea Advanced Institute of Science and Technology
Document Type
Article
Publication Date
4-1-2015
Abstract
The Drosophila eye-antenna imaginal disc (ead) is a flattened sac of two-layered epithelia, from which most head structures are derived. Secreted morphogens like Wingless (Wg), Hedgehog (Hh), and Decapentaplegic (Dpp) are important for early patterning of ead, but the underlying mechanisms are still largely unknown. To understand how these morphogens function in the ead of early larval stages, we used wg-LacZ and dpp-Gal4 markers for the examination of wild-type and mutant eads. We found that the ead immediately after hatching was crescent-shaped with the Bolwig’s nerve at the ventral edge, suggesting that it consists of dorsal domain. In a subsequent step, transcriptional induction of dpp in the cells along the Bolwig’s nerve was followed by rapid growth of the ventral domain. Both Wg and Hh were required for the formation of the ventral domain. Wg was crucial for the growth of the entire ead, but Hh was essential for cell division only in the dorsal domain. In the ventral domain, Hh regulated dpp transcription. Based on these data, we propose that signaling among distinct groups of cells expressing Wg, Dpp, or Hh in the ead of the first-instar larvae are critical for coordinated growth and patterning of ead.
Inclusive pages
1-18
ISBN/ISSN
1932-6203
Document Version
Published Version
Comments

This document is provided for download in compliance with the publisher's policy on self-archiving. Permission documentation is on file.

Publisher
PLoS
Peer Reviewed
Yes
Citation Information
Jong-Hoon Won, Orkhon Tsogtbartarr, Wonseok Son, Amit Singh, et al.. "Cell Type-Specific Responses to Wingless, Hedgehog and Decapentaplegic Are Essential for Patterning Early Eye-Antenna Disc in Drosophila" PLOS One Vol. 10 Iss. 4 (2015)
Available at: http://works.bepress.com/amit_singh/42/