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Article
Domain Specific Genetic Mosaic System in the Drosophila Eye
Genesis: The Journal of Genetics and Development
  • Meghana Tare, University of Dayton
  • Oorvashi Roy Puli, University of Dayton
  • Michael Moran, University of Dayton
  • Madhuri Kango-Singh, University of Dayton
  • Amit Singh, University of Dayton
Document Type
Article
Publication Date
1-1-2013
Abstract
Genetic mosaic approach is commonly used in the Drosophila eye by completely abolishing or misexpressing a gene within a subset of cells to unravel its role during development. Classical genetic mosaic approach involves random clone generation in all developing fields. Consequently, a large sample size needs to be screened to generate and analyze clones in specific domains of the developing eye. To address domain specific functions of genes during axial patterning, we have developed a system for generating mosaic clones by combining Gal4/UAS and flippase (FLP)/FRT system which will allow generation of loss-of-function as well as gain-of-function clones on the dorsal and ventral eye margins. We used the bifid-Gal4 driver to drive expression of UAS-FLP. This reagent can have multiple applications in (i) studying spatio-temporal function of a gene during dorso-ventral (DV) axis specification in the eye, (ii) analyzing genetic epistasis of genes involved in DV patterning, and (iii) conducting genome wide screens in a domain specific manner.
Inclusive pages
68–74
ISBN/ISSN
1526-954X
Comments

Permission documentation is on file.

Publisher
Wiley Periodicals
Peer Reviewed
Yes
Citation Information
Meghana Tare, Oorvashi Roy Puli, Michael Moran, Madhuri Kango-Singh, et al.. "Domain Specific Genetic Mosaic System in the Drosophila Eye" Genesis: The Journal of Genetics and Development Vol. 51 Iss. 1 (2013)
Available at: http://works.bepress.com/amit_singh/36/