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Article
Protein Trap Lines of Drosophila to demonstrate Spatio-Temporal Localization of Proteins in an Undergraduate Lab
Drosophila Information Service
  • Oorvashi Roy Puli, University of Dayton
  • Amit Singh, University of Dayton
Document Type
Article
Publication Date
12-1-2011
Abstract
The objective of this teaching note is to generate a laboratory exercise, which allows students to get a hands-on experience of a cell biology technique. The short duration of the laboratory classes is the biggest challenge with the development of a cell biology lab for an undergraduate curriculum. Therefore, it is necessary to design a laboratory exercise that enables the students to carry out cell biological assays in the desired time. This laboratory exercise focuses on tracking protein expression levels along a spatial (space) and temporal (time) axis in developing Drosophila melanogaster organ primordium. Here we use the protein trap model developed in Drosophila to demonstrate the sub-cellular localization of proteins. The protein trap transgenic flies have green fluorescent protein (GFP) reporter tags to the full-length endogenous proteins that allow observation of their cellular as well as sub-cellular distribution. Since the life cycle of Drosophila is short, it is easy to rear them in the lab and also use them as an excellent model for an undergraduate lab curriculum. The goal of this exercise is to train undergraduate students and teach them the use of one such powerful tool which enables the localization of proteins.
Inclusive pages
190-196
ISBN/ISSN
0070-7333
Document Version
Published Version
Comments

This document is provided for download by permission of the publisher. Permission documentation is on file.

Publisher
University of Oklahoma
Peer Reviewed
Yes
Citation Information
Oorvashi Roy Puli and Amit Singh. "Protein Trap Lines of Drosophila to demonstrate Spatio-Temporal Localization of Proteins in an Undergraduate Lab" Drosophila Information Service Vol. 94 (2011)
Available at: http://works.bepress.com/amit_singh/35/