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Article
Production of nitrogen-based platform chemical: cyanophycin biosynthesis using recombinant Escherichia coli and renewable media substitutes
Journal of Chemical Technology and Biotechnology (2013)
  • Amit Kumar, Kansas State University
Abstract

BACKGROUND:Cyanophycin (CGP) is a sustainable polymer that can be converted to a derivative with reduced arginine content, or to completely biodegradable poly-aspartic acid, which can substitute for non-biodegradable polyacrylates. In nature, it is produced by most cyanobacteria; however, these microbes are not suitable for large-scale production due to slow growth and low polymer content. RESULTS: Cyanophycin synthetase gene (cphA) from Anabaena variabilis ATCC 29413 was PCR amplified and cloned into Escherichiacoli.Differentrenewablemediacomponents,suchassoybeanmeal,potatowastesandcorn-derivedzein hydrolysate, were evaluated for their feasibility for CGP production at shake flask level. The optimized conditions were then tested in a 7 L bioreactor; a maximum cell weight of 10.2 g L-1 was obtained. CGP comprised 23 g g-1 of cell dry matter (CDM) with molecular weight between 21.5 and 31 kDa and was composed of aspartic acid, arginine and lysine in the ratio of 1.05:1:0.2 (mass basis). CONCLUSION: The efficient use of renewable biomass for cyanophycin production could achieve competitive price, and in return, promote the use of this platform chemical to produce innovative polymers and materials.

Keywords
  • cyanophycin; fermentation; platform chemical; recombinant E.coli; amino acids
Disciplines
Publication Date
2013
Citation Information
Amit Kumar. "Production of nitrogen-based platform chemical: cyanophycin biosynthesis using recombinant Escherichia coli and renewable media substitutes" Journal of Chemical Technology and Biotechnology Vol. 88 Iss. 7 (2013)
Available at: http://works.bepress.com/amit_kumar/3/