"Bismuth–thiol incorporation enhances biological activities of liposomal tobramycin against bacterial biofilm and quorum sensing molecules production by Pseudomonas aeruginosa" by Majed Halwani

Article

Bismuth–thiol incorporation enhances biological activities of liposomal tobramycin against bacterial biofilm and quorum sensing molecules production by Pseudomonas aeruginosa

International Journal of Pharmaceutics (2009)

Majed Halwani
Stephanie Hebert
Zacharias E. Suntres
Robert M. Lafrenie
Ali Azghani, University of Texas at Tyler
Abdelwahab Omri

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Abstract

Recurrent pulmonary infection and inflammation are major risk factors for high morbidity and mortality in patients with cystic fibrosis (CF). As such, frequent antibiotic use and drug resistant bacterial strains are main concerns in individuals with CF. Bacterial virulence and resistance are influenced by unique CF airways fluid lining andPseudomonas aeruginosa quorum sensing (QS) and biofilm formation. We have developed a novel liposome formulation consist of bismuth–thiol and tobramycin (LipoBiEDT–TOB) that is non-toxic and highly effective against planktonic bacteria. In this study, we examined the effect of LipoBiEDT–TOB on QS molecule N-acyl homoserine lactone (AHL) secretion by P. aeruginosa isolates in the presence ofAgrobacterium tumefaciens reporter strain (A136). LipoBiEDT–TOB activity against biofilm forming P. aeruginosa was compared to free tobramycin using the Calgary Biofilm Device (CBD). Our data indicate that LipoBiEDT–TOB prevents AHL production at low tobramycin concentration (as low as 0.012 mg/l) and stops biofilm forming P. aeruginosagrowth at 64 mg/l. The formulation is stable in different biological environments (biofilm, sputum, and bronchoalveolar lavage) and is able to penetrate CF sputum. Taken together, co-encapsulation of bismuth–thiol metal with tobramycin in liposome improves its antimicrobial activities in vitro.

Keywords

Liposomes,
Tobramycin,
Bismuth,
Cystic fibrosis,
Quorum sensing,
Sputum

Disciplines

Biology

Publication Date

May, 2009

DOI

http://dx.doi.org/10.1016/j.ijpharm.2009.02.001

Citation Information

Halwani M, Hebert S, Suntres ZE, Lafrenie RM, Azghani AO, Omri A. 2009. Bismuththiol incorporation enhances biological activities of liposomal tobramycin against bacterial biofilm and quorum sensing molecules production by Pseudomonas aeruginosa. Int J Pharm. 21;373(1-2): 141-6.