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Molecular characterisation of canine nonsteroidal anti-inflammatory drug-activated gene (NAG-1)
Veterinary Journal (2008)
  • K Yamaguchi, University of Tennessee - Knoxville
  • Nichelle C Whitlock, University of Tennessee - Knoxville
  • Jason L Liggett, University of Tennessee - Knoxville
  • Alfred M Legendre, University of Tennessee
  • Michael M Fry, University of Tennessee - Knoxville
  • Seung J Baek
Abstract

Nonsteroidal anti-inflammatory drug (NSAID)-activated gene (NAG-1), a divergent member of the transforming growth factor beta superfamily, was previously identified as a gene induced by several anti-tumorigenic compounds, including NSAIDs and peroxisome proliferator-activated receptor gamma (PPARgamma) ligands in humans. In this study, canine NAG-1 was characterised from a canine genomic database. Gene induction by some NSAIDs and PPARgamma ligands was demonstrated in canine osteosarcoma cell lines. Phylogenetic analysis indicates that canine NAG-1 is more homologous with the corresponding mouse and rat genes than with human NAG-1. Expression of canine NAG-1 was increased by treatment with piroxicam and SC-560 (NSAIDs) and the PPARgamma ligand rosiglitazone. This study demonstrates that canine NAG-1 is up-regulated by some anti-tumorigenic compounds in osteosarcoma cell lines and may provide an important target of chemotherapy in canine cancer.

Keywords
  • Amino Acid Sequence,
  • Animals,
  • Anti-Inflammatory Agents,
  • Non-Steroidal pharmacology,
  • Non-Steroidal therapeutic use,
  • Cell Line,
  • Tumor,
  • Cytokines genetics,
  • Dogs,
  • Gene Expression Regulation drug effects,
  • Growth Differentiation Factor 15,
  • Humans,
  • Mice,
  • Molecular Sequence Data,
  • Osteosarcoma drug therapy,
  • veterinary,
  • PPAR gamma,
  • PPAR gamma/therapeutic use,
  • Phylogeny,
  • RNA,
  • Rats,
  • Sequence Alignment,
  • Species Specificity,
  • Transcriptional Activation,
  • Up-Regulation
Publication Date
January, 2008
Citation Information
K Yamaguchi, Nichelle C Whitlock, Jason L Liggett, Alfred M Legendre, et al.. "Molecular characterisation of canine nonsteroidal anti-inflammatory drug-activated gene (NAG-1)" Veterinary Journal Vol. 175 Iss. 1 (2008)
Available at: http://works.bepress.com/alfred_legendre/1/