A simple approach to test the ability of bacteria to undergo natural genetic transformation is suggested. The basic feature of the approach is the cultivation of bacterial cells in the presence of exogenous (plasmid) DNA on cellphone membranes placed successively on nutrient and selective agar. Using this approach the ability of Bacillus cereus for “natural” genetic transformation was detected. Transformation frequencies varied from 10 E−8 to 10 E−6.
- Transformation; Bacillus cereus; Plasmid
Available at: http://works.bepress.com/alexander_sabelnikov/14/