Article
Silica Adsorbents with well-defined Peracetylated Thiosaccharide Surfaces: Synthesis and use for Stereoselective Separations in RP and NP HPLC
Chromatographia
(2013)
Abstract
New chiral silica adsorbents with well-defined peracetylated thiosaccharide (a.k.a. thiosaccharide) surfaces containing 1, 3, 7, and 13 glucose units have been synthesized through the reaction of trimethoxysilyl-derivatives of thiosaccharides with bare silica gel. As determined by FTIR, nitrogen adsorption, thermo-gravimetric analysis, and chemical analysis, the adsorbents prepared contained closely packed uniform monolayer surfaces of thiosaccharide groups grafted to silica. The chromatographic behavior of the thiosaccharide-silicas prepared has been evaluated in the HPLC separations of stereoisomers in both normal and reversed phase modes. With the exception of low grafted thiomaltodecatriose-silica, there was no effect of the size of thiosaccharide on the selectivity of the separations.
Keywords
- Chiral silica adsorbents,
- Column liquid chromatography,
- Immobilization,
- Peracetylated thiosaccharides,
- Silica surface,
- Stereoisomers separation,
- Stereoselectivity
Disciplines
Publication Date
December, 2013
DOI
10.1007/s10337-013-2549-9
Citation Information
Alexander Y. Fadeev, Yuri V. Kazakevich, Cecilia H. Marzabadi and Edwin Vega. "Silica Adsorbents with well-defined Peracetylated Thiosaccharide Surfaces: Synthesis and use for Stereoselective Separations in RP and NP HPLC" Chromatographia Vol. 76 Iss. 23-24 (2013) p. 1585 - 1593 Available at: http://works.bepress.com/alexander_fadeev/10/