Using meteor radar, radiosonde observations and MERRA-2 reanalysis data from 12 August to 31 October 2006, we report a dynamical coupling from the tropical lower atmosphere to the mesosphere and lower thermosphere through a quasi-27-day intraseasonal oscillation (ISO). It is interesting that the quasi-27-day ISO is observed in the troposphere, stratopause and mesopause regions, exhibiting a three-layer structure. In the MLT, the amplitude in the zonal wind increases from about 4 ms−1 at 90 km to 15 ms−1 at 100 km, which is diferent from previous observations that ISOs occurs generally in winter with an amplitude peak at about 80–90 km, and then are rapidly weakened with increasing height. Outgoing longwave radiation (OLR) and specifc humidity demonstrate that there is a quasi-27-day periodicity in convective activity in the tropics, which causes the ISO of the zonal wind and gravity wave (GW) activity in the troposphere. The upward propagating GWs are further modulated by the oscillation in the troposphere and upper stratosphere. As the GWs propagate to the MLT, the quasi-27-day oscillation in the wind feld is induced with a clear phase opposite to that in the lower atmosphere through instability and dissipation of these modulated GWs. Wavelet analysis shows that the quasi-27-day variability in the MLT appears as a case event rather than a persistent phenomenon, and has not a clear corresponding relation with the solar rotation efect within 1 year of observations.