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Article
Quantitation of CD8+ T cell responses to newly identified HLA-A*0201-restricted T cell epitopes conserved among vaccinia and variola (smallpox) viruses
Open Access Articles
  • Masanori Terajima, University of Massachusetts Medical School
  • John Cruz, University of Massachusetts Medical School
  • Gregory Raines
  • Elizabeth D. Kilpatrick, University of Massachusetts Medical School
  • Jeffrey S. Kennedy
  • Alan L. Rothman, University of Massachusetts Medical School
  • Francis A. Ennis, University of Massachusetts Medical School
UMMS Affiliation
Center for Infectious Disease and Vaccine Research
Date
4-2-2003
Document Type
Article
Subjects
CD8-Positive T-Lymphocytes; Cell Line; *Epitopes, T-Lymphocyte; HLA-A Antigens; Humans; Immunologic Memory; Smallpox Vaccine; T-Lymphocytes, Cytotoxic; Vaccinia virus
Abstract

Immunization with vaccinia virus resulted in long-lasting protection against smallpox and was the approach used to eliminate natural smallpox infections worldwide. Due to the concern about the potential use of smallpox virus as a bioweapon, smallpox vaccination is currently being reintroduced. Severe complications from vaccination were associated with congenital or acquired T cell deficiencies, but not with congenital agammaglobulinemia, suggesting the importance of T cell immunity in recovery from infection. In this report, we identified two CD8+ T cell epitopes restricted by the most common human major histocompatibility complex (MHC) class I allele, HLA-A*0201. Both epitopes are highly conserved in vaccinia and variola viruses. The frequency of vaccinia-specific CD8+ T cell responses to these epitopes measured by interferon (IFN)-gamma enzyme-linked immunospot (ELISPOT) assay and HLA/peptide tetramer staining peaked 2 wk after primary immunization and then declined, but were still detectable 1 to 3 yr after primary immunization. 2 wk after immunization, IFN-gamma-producing cells specific to these two epitopes were 14% of total vaccinia virus-specific IFN-gamma-producing cells in one donor, 35% in the second donor, and 6% in the third donor. This information will be useful for studies of human T cell memory and for the design and analyses of the immunogenicity of experimental vaccinia vaccines.

Rights and Permissions
Citation: J Exp Med. 2003 Apr 7;197(7):927-32. Epub 2003 Mar 31. Link to article on publisher's site
Related Resources
Link to Article in PubMed
PubMed ID
12668642
Citation Information
Masanori Terajima, John Cruz, Gregory Raines, Elizabeth D. Kilpatrick, et al.. "Quantitation of CD8+ T cell responses to newly identified HLA-A*0201-restricted T cell epitopes conserved among vaccinia and variola (smallpox) viruses" Vol. 197 Iss. 7 (2003) ISSN: 0022-1007 (Print)
Available at: http://works.bepress.com/alan_rothman/16/