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Article
Quantitation of CD8+ T cell responses to newly identified HLA-A*0201-restricted T cell epitopes conserved among vaccinia and variola (smallpox) viruses
Open Access Articles
  • Masanori Terajima, University of Massachusetts Medical School
  • John Cruz, University of Massachusetts Medical School
  • Gregory Raines
  • Elizabeth D. Kilpatrick, University of Massachusetts Medical School
  • Jeffrey S. Kennedy
  • Alan L. Rothman, University of Massachusetts Medical School
  • Francis A. Ennis, University of Massachusetts Medical School
UMMS Affiliation
Center for Infectious Disease and Vaccine Research
Date
4-2-2003
Document Type
Article
Subjects
CD8-Positive T-Lymphocytes; Cell Line; *Epitopes, T-Lymphocyte; HLA-A Antigens; Humans; Immunologic Memory; Smallpox Vaccine; T-Lymphocytes, Cytotoxic; Vaccinia virus
Abstract

Immunization with vaccinia virus resulted in long-lasting protection against smallpox and was the approach used to eliminate natural smallpox infections worldwide. Due to the concern about the potential use of smallpox virus as a bioweapon, smallpox vaccination is currently being reintroduced. Severe complications from vaccination were associated with congenital or acquired T cell deficiencies, but not with congenital agammaglobulinemia, suggesting the importance of T cell immunity in recovery from infection. In this report, we identified two CD8+ T cell epitopes restricted by the most common human major histocompatibility complex (MHC) class I allele, HLA-A*0201. Both epitopes are highly conserved in vaccinia and variola viruses. The frequency of vaccinia-specific CD8+ T cell responses to these epitopes measured by interferon (IFN)-gamma enzyme-linked immunospot (ELISPOT) assay and HLA/peptide tetramer staining peaked 2 wk after primary immunization and then declined, but were still detectable 1 to 3 yr after primary immunization. 2 wk after immunization, IFN-gamma-producing cells specific to these two epitopes were 14% of total vaccinia virus-specific IFN-gamma-producing cells in one donor, 35% in the second donor, and 6% in the third donor. This information will be useful for studies of human T cell memory and for the design and analyses of the immunogenicity of experimental vaccinia vaccines.

Rights and Permissions
Citation: J Exp Med. 2003 Apr 7;197(7):927-32. Epub 2003 Mar 31. Link to article on publisher's site
DOI of Published Version
10.1084/jem.20022222
Related Resources
Link to Article in PubMed
PubMed ID
12668642
Citation Information
Masanori Terajima, John Cruz, Gregory Raines, Elizabeth D. Kilpatrick, et al.. "Quantitation of CD8+ T cell responses to newly identified HLA-A*0201-restricted T cell epitopes conserved among vaccinia and variola (smallpox) viruses" Vol. 197 Iss. 7 (2003) ISSN: 0022-1007 (Print)
Available at: http://works.bepress.com/alan_rothman/16/