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Low-Pathogenic Avian Influenza Viruses in Wild House Mice
USDA National Wildlife Research Center - Staff Publications
  • Susan A. Shriner, USDA Wildlife Services
  • Kaci Van Dalen, US Department of Agriculture
  • Nicole L. Mooers, United States Department of Agriculture (USDA)
  • Jeremy W. Ellis, United States Department of Agriculture (USDA)
  • Heather J. Sullivan, United States Department of Agriculture (USDA)
  • J. Jeffrey Root, National Wildlife Research Center
  • Angela M. Pelzel, United States Department of Agriculture (USDA)
  • Alan B. Franklin, USDA-APHIS, Wildlife Services’ National Wildlife Research Center
Date of this Version

PLoS ONE 7(6): e39206; doi:10.1371/journal.pone.0039206

Background: Avian influenza viruses are known to productively infect a number of mammal species, several of which are commonly found on or near poultry and gamebird farms. While control of rodent species is often used to limit avian influenza virus transmission within and among outbreak sites, few studies have investigated the potential role of these species in outbreak dynamics. Methodology/Principal Findings: We trapped and sampled synanthropic mammals on a gamebird farm in Idaho, USA that had recently experienced a low pathogenic avian influenza outbreak. Six of six house mice (Mus musculus) caught on the outbreak farm were presumptively positive for antibodies to type A influenza. Consequently, we experimentally infected groups of naïve wild-caught house mice with five different low pathogenic avian influenza viruses that included three viruses derived from wild birds and two viruses derived from chickens. Virus replication was efficient in house mice inoculated with viruses derived from wild birds and more moderate for chicken-derived viruses. Mean titers (EID50 equivalents/mL) across all lung samples from seven days of sampling (three mice/day) ranged from 103.89 (H3N6) to 105.06 (H4N6) for the wild bird viruses and 102.08 (H6N2) to 102.85 (H4N8) for the chicken-derived viruses. Interestingly, multiple regression models indicated differential replication between sexes, with significantly (p<0.05) higher concentrations of avian influenza RNA found in females compared with males. Conclusions/Significance: Avian influenza viruses replicated efficiently in wild-caught house mice without adaptation, indicating mice may be a risk pathway for movement of avian influenza viruses on poultry and gamebird farms. Differential virus replication between males and females warrants further investigation to determine the generality of this result in avian influenza disease dynamics.
Citation Information
Susan A. Shriner, Kaci Van Dalen, Nicole L. Mooers, Jeremy W. Ellis, et al.. "Low-Pathogenic Avian Influenza Viruses in Wild House Mice" (2012)
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