The molecular effects of chronic heat stress on expression profiles of insulin- and phosphatidylinositol-3 kinase (PI3K)-mediated steroidogenesis signaling pathway components were evaluated in gilt ovaries. Comprehending the molecular mechanism by which heat stress compromises pig reproductive performance is of substantial relevance and such knowledge will be indispensable in developing strategies and therapeutics to mitigate these negative effects. Our results indicate that after 35 days of thermal stress, expression of PI3K signaling pathway members is significantly altered, which could impact follicle activation, affect follicle viability and potentially modify ovarian steroid synthesis, thus leading to negative impacts on fertility in swine.