: In this study, sediments samples from two reservoirs; King Talal and Al-Mujib/Jordan were collected to assess their effect on growth, yield and genetic integrity of Spinach and Chrysanthemum. Soil samples from Irhab area were used as control, Irhab soil was mixed with sediments collected from two areas from each dam (Near and far from the dam wall) in different levels (15 or 30% sediments (w/w)). Results show that growing Chrysanthemum plants in soil supplemented with 30% sediment collected from KTD (Near) resulted in the maximum plant growth and yield compared to control or plants received organic or inorganic fertilizers. Whereas, Spinach plants grown in soil supplemented with sediments collected from KTD, organic or inorganic fertilizers showed similar fresh weight. Results of Comet assay showed that no DNA damage was detected for plants grown either in control soil or in soil supplemented with different dam sediments. ISSR analysis showed similar DNA fingerprinting profile between Spinach plants either grown in soil alone or in soil supplemented with sediments indicating that soil supplementation with dam sediments is not genotoxic. Heavy metals analysis indicated no significant effect of adding sediments to growing soil of Chrysanthemum but significant higher levels of Fe, Cu, Zn, Cd and Pb were observed in Spinach plants. In conclusion, the findings of this study present evidence for the potential use of King Talal and Al-Mujib dam's sediments for soil supplementation for Chrysanthemum in areas with low soil fertility and as a mean of restoring reservoir storage capacity