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Article
Retrospective analysis of adverse reactions associated with 230 allogenic administrations of bone marrow-derived mesenchymal stem cells in 164 horses
Equine Veterinary Journal (2018)
  • Tena Ursini
  • Lisa L Amelse, University of Tennessee - Knoxville
  • Hoda Elkhenany
  • Agricola Odoi
  • Jessica Carter-Arnold
  • Steve Adair
  • Madhu Dhar, University of Tennessee - Knoxville
Abstract
Background
Bone marrow‐derived mesenchymal stem cells (BM‐MSCs) are frequently used in the treatment of musculoskeletal injuries. Fully characterised cells that are readily available for use is optimum. Allogenic BM‐MSCs can satisfy the need for rapid treatment, however, their safety has been questioned.
Objectives
Objectives were to characterise BM‐MSCs from an adult donor horse, in vitro, and to identify and describe adverse reactions that occurred following their injection into other horses. We hypothesised that BM‐MSCs capable of proliferation, differentiation and lacking MHC II from one donor could be implanted into another individual without significant adverse reactions and the frequency of adverse reactions in clinical cases would be similar to that previously reported for autologous BM‐MSCs.
Study design
Retrospective clinical study.
Methods
BM‐MSCs were proliferated and characterised from one donor and cryopreserved for clinical use. Medical records for horses injected with allogenic BM‐MSCs from this donor at a single hospital were used. After routine lameness exam, lesions were identified using diagnostic ultrasound or MRI. Post injection reaction was defined as increased pain, swelling, or heat at or near injection site, or increased lameness. Treatments required for each reaction were noted.
Results
BM‐MSCs proliferated and underwent differentiation. Cells were found to be negative for MHC‐II (<2%) and were viable after cryopreservation and shipping. Ten of 230 (4.35%) injections were noted to be associated with an adverse reaction. Adverse reactions occurred in synovial structures (n = 3) and in soft tissues (n = 7).
Main limitations
This investigation could underestimate the number and severity of reactions. Mild reactions, such as synovitis, may have been missed. Also, anti‐inflammatory drugs could overshadow mild reactions, making them less likely to be detected.
Conclusions
Fully characterised allogenic BM‐MSCs originating from a single donor horse can be administered to horses with soft tissue injuries with a low rate of adverse reaction.
Disciplines
Publication Date
July 10, 2018
DOI
https://doi.org/10.1111/evj.12992
Citation Information
Tena Ursini, Lisa L Amelse, Hoda Elkhenany, Agricola Odoi, et al.. "Retrospective analysis of adverse reactions associated with 230 allogenic administrations of bone marrow-derived mesenchymal stem cells in 164 horses" Equine Veterinary Journal (2018)
Available at: http://works.bepress.com/agricola_odoi/63/