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Article
An in vivo model for investigating bilateral synaptic plasticity across CA3/CA1 synapses in guinea pig dorsal hippocampus
Journal of neuroscience methods
  • S. Chirwa
  • J. Mack
  • R. Park
  • K. Dennis
  • Adwoa Aduonum, Philadelphia College of Osteopathic Medicine
Document Type
Article
Publication Date
1-1-2001
Abstract
A method is described for concurrent investigation of long-term potentiation (LTP) in the left and right CA1 synapses in dorsal hippocampi in guinea pig in vivo. Briefly, animals are anesthetized with urethane, and small access holes are made in the skull through which electrodes are lowered to stimulate the left CA3 and record from both CA1 regions. Using this animal model, we have found that LTP is produced in both CA1 regions, following conditioning stimulation to the left CA3. However, in some animals LTP occurred in the left CA1 without concomitant synaptic potentiation in the contralateral CA1. We also observed that in some experiments synaptic potentiation in the contralateral CA1, when present, decayed to baseline levels even though LTP persisted in the ipsilateral CA1. To conclude, our data on bilateral LTP demonstrates findings that are best addressed in vivo. © 2001 Elsevier Science B.V. All rights reserved.
Comments

This article was published in Journal of neuroscience methods, Volume 110, Issue 42006, Pages 25-30.

The published version is available at http://dx.doi.org/10.1016/S0165-0270(01)00413-7.

Copyright © 2001 Scopus.

Citation Information
S. Chirwa, J. Mack, R. Park, K. Dennis, et al.. "An in vivo model for investigating bilateral synaptic plasticity across CA3/CA1 synapses in guinea pig dorsal hippocampus" Journal of neuroscience methods Vol. 110 Iss. 42006 (2001) p. 25 - 30
Available at: http://works.bepress.com/adwoa_aduonum/3/