The HIV protease-reverse transcriptase (PR-RT) (1047 bp), gp120-env (891 bp) and gp41-env (547 bp) regions from the plasma of 115 HIV-1-infected patients in Kuala Lumpur (KL), Malaysia were sequenced. Detailed phylogenetic and bootscanning analyses were performed to determine the mosaic structure of the HIV-1 strains and their recombination breakpoint(s). Among the 50 patient samples in which all three regions could be amplified, the HIV-1 CRF0 1_AE subtype (46%) was predominant followed by subtypes B (10%) and B' (6%). A total of 9/50 (18%) patients were infected with a CR-F01_AE/B inter-subtype recombinant, displaying a recombinant form (RF) PR-RT, CPF01__AE(gp120-env) and CRF01_AE(gp41-evn). This RF was derived from the Thai variants of CRF01_AE and B' subtype, with two distinct B' subtype segments in the backbone of CRF01_AE, similar to the newly identified CRF33-01 B. In addition, one sample demonstrated a close structural relationship with the new CRF33_01B in the PR-RT region but displayed B' segment in part of the env region (RFPR-RT, CRF01_AE/B'(gp120-env)) and B'(gp41-env)) indicating continuing evolution of CRF33_01B. The remaining 18% of samples were identified as unique recombinant forms (URFs). (c) 2007 Elsevier Inc. All rights reserved.
- Malaysia; HIV; AIDS; Recombination; Circulating recombinant forms; Epidemiology
Available at: http://works.bepress.com/adeeba_kamarulzaman/2/