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Selected Works of Zengyi Shao
Article
Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler
Molecular BioSystems (2011)
  • Zengyi Shao, University of Illinois at Urbana-Champaign
  • Yunzi Luo, University of Illinois at Urbana-Champaign
  • Huimin Zhao, University of Illinois at Urbana-Champaign
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Abstract

We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unprecedented flexibility and versatility in pathway manipulations.

Keywords
  • Bacterium,
  • biosynthesis,
  • gene vecor,
  • genetic engineering,
  • genetic recombination,
  • genetics,
  • metabolism,
  • Saccharomyces cerevisiae,
  • synthetic biology,
  • Institute for Genomic Biology
Disciplines
Publication Date
2011
Publisher Statement
Copyright The Royal Society of Chemistry 2011. Posted with permission.
Citation Information
Zengyi Shao, Yunzi Luo and Huimin Zhao. "Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler" Molecular BioSystems Vol. 7 Iss. 4 (2011)
Available at: http://works.bepress.com/zengyi_shao/4/