Skip to main content
Article
Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenases Interact with Phospholipase Dδ to Transduce Hydrogen Peroxide Signals in the Arabidopsis Response to Stress
The Plant Cell (2012)
  • Xuemin Wang, University of Missouri-St. Louis
  • Liang Guo, University of Missouri–St. Louis
  • Shivakumar P. Devaiah
  • Rama Narasimhan
  • Xiangqing Pan
  • Yanyan Zhang, University of Missouri-St. Louis
  • Wenhua Zhang, Nanjing Agricultural University
Abstract
Reactive oxygen species (ROS) are produced in plants under various stress conditions and serve as important mediators in plant responses to stresses. Here, we show that the cytosolic glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenases (GAPCs) interact with the plasma membrane–associated phospholipase D (PLDδ) to transduce the ROS hydrogen peroxide (H2O2) signal in Arabidopsis thaliana. Genetic ablation of PLDδ impeded stomatal response to abscisic acid (ABA) and H2O2, placing PLDδ downstream of H2O2 in mediating ABA-induced stomatal closure. To determine the molecular link between H2O2 and PLDδ, GAPC1 and GAPC2 were identified to bind to PLDδ, and the interaction was demonstrated by coprecipitation using proteins expressed in Escherichia coli and yeast, surface plasmon resonance, and bimolecular fluorescence complementation. H2O2 promoted the GAPC–PLDδ interaction and PLDδ activity. Knockout of GAPCs decreased ABA- and H2O2-induced activation of PLD and stomatal sensitivity to ABA. The loss of GAPCs or PLDδ rendered plants less responsive to water deficits than the wild type. The results indicate that the H2O2-promoted interaction of GAPC and PLDδ may provide a direct connection between membrane lipid–based signaling, energy metabolism and growth control in the plant response to ROS and water stress.
Disciplines
Publication Date
May, 2012
DOI
10.1105/tpc.111.094946
Publisher Statement
© American Society of Plant Biologists.
Citation Information
Xuemin Wang, Liang Guo, Shivakumar P. Devaiah, Rama Narasimhan, et al.. "Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenases Interact with Phospholipase Dδ to Transduce Hydrogen Peroxide Signals in the Arabidopsis Response to Stress" The Plant Cell Vol. 24 (2012) p. 2200 - 2212
Available at: http://works.bepress.com/xuemin-wang/49/