Article
An electrochemical super sandwich assay for sensitive and selective DNA detection in complex matrices
Polymer Engineering Faculty Research
Document Type
Article
Publication Date
10-1-2010
Disciplines
Abstract
In a traditional sandwich assay, a DNA target hybridizes to a single copy of the signal probe. Here we employ a modified signal probe containing a methylene blue (a redox moiety) label and a “sticky end.” When a DNA target hybridizes this signal probe, the sticky end remains free to hybridize another target leading to the creation of a supersandwich structure containing multiple labels. This leads to large signal amplification upon monitoring by voltammetry.
Citation Information
Xiong Gong. "An electrochemical super sandwich assay for sensitive and selective DNA detection in complex matrices" Vol. 132 (2010) p. 14346 Available at: http://works.bepress.com/xiong_gong/121/