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Article
Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays
Proceedings of the National Academy of Sciences of the United States of America (2009)
  • A Bajaj
  • OR Miranda
  • IB Kim
  • RL Phillips
  • DJ Jerry
  • UHF Bunz
  • VM Rotello
Abstract

Rapid and effective differentiation between normal and cancer cells is an important challenge for the diagnosis and treatment of tumors. Here, we describe an array-based system for identification of normal and cancer cells based on a “chemical nose/tongue” approach that exploits subtle changes in the physicochemical nature of different cell surfaces. Their differential interactions with functionalized nanoparticles are transduced through displacement of a multivalent polymer fluorophore that is quenched when bound to the particle and fluorescent after release. Using this sensing strategy we can rapidly (minutes/seconds) and effectively distinguish (i) different cell types; (ii) normal, cancerous and metastatic human breast cells; and (iii) isogenic normal, cancerous and metastatic murine epithelial cell lines.

Keywords
  • fluorescence,
  • gold nanoparticle,
  • sensor,
  • conjugated polymer
Disciplines
Publication Date
June 23, 2009
Publisher Statement
doi: 10.1073/pnas.0900975106
Citation Information
A Bajaj, OR Miranda, IB Kim, RL Phillips, et al.. "Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays" Proceedings of the National Academy of Sciences of the United States of America Vol. 106 Iss. 27 (2009)
Available at: http://works.bepress.com/vincent_rotello/24/