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Article
Intracellular Delivery of a Membrane-Impermeable Enzyme in Active Form Using Functionalized Gold Nanoparticles
Journal of the American Chemical Society
(2010)
Abstract
Gold nanoparticles were coated with a short peptide to promote intracellular delivery of membrane-impermeable proteins. Through microscopy and enzyme assays, we demonstrated the particles were able to transport functional enzymes into a variety of cell lines. Significantly, the transported proteins were able to escape from endosomes. Moreover, these particles showed no apparent cytotoxicity.
Disciplines
Publication Date
February 4, 2010
Publisher Statement
DOI: 10.1021/ja907887z
Citation Information
P Ghosh, XC Yang, R Arvizo, ZJ Zhu, et al.. "Intracellular Delivery of a Membrane-Impermeable Enzyme in Active Form Using Functionalized Gold Nanoparticles" Journal of the American Chemical Society Vol. 132 Iss. 8 (2010) Available at: http://works.bepress.com/vincent_rotello/18/