Development of real–time PCR assays for the detection of Chattonella species in culture and environmental samples
Raphidophytes have been associated with fish kill events in Japanese, European and U.S. coastal waters, and many produce toxins that can pose a threat to human health. The recognition of raphidophytes as HAB species in mid-Atlantic estuarine waters led us to initiate molecular phylogenetic analyses of these taxa and to develop real-time PCR assays for rapid detection of important species. The molecular studies and PCR detection methods will enhance ongoing taxonomic, toxicologic and ecological of these organisms and will be a useful tool in HAB monitoring programs.
Bowers, H., Tengs, T., Goto, S., Tomas, C., Ono, C., Yoshimatsu, S. and Oldach, D. (2004) Development of Real–Time PCR assays for the detection of Chattonella species in culture and environmental samples. In: Steidinger, K.A., Landsberg, J.H., Tomas, C.R. and Vargo, G.A. (eds.), Harmful Algae 2002. Florida Fish and Wildlife Conservation Comission, Florida Oceanography and Intergovernmental Oceanography Comission of UNESCO, pp. 231–233.