Cancer
A transforming MET mutation discovered in non-small cell lung cancer using microarray-based resequencing, Cancer Letters (2006)
We have designed resequencing microarrays to test the performance of this platform when interrogating a...
Sensitive mutation detection in heterogeneous cancer specimens by massively parallel picoliter reactor sequencing, Nature Medicine (2006)
The sensitivity of conventional DNA sequencing in tumor biopsies is limited by stromal contamination and...
Detection of oncogenic mutations in the EGFR gene in lung adenocarcinoma with differential sensitivity to EGFR tyrosine kinase inhibitors, Cold Spring Harbor Symposia on Quantitative Biology (2005)
The complete sequencing of the human genome and the development of molecularly targeted cancer therapy...
Computational Biology/Bioinformatics
UniquePrimer - a web utility for design of specific PCR primers and probes, Annals of Microbiology (2009)
We have developed a web-based tool for design of specific PCR primers and probes. The...
Statistical analysis of genomic tag data, Statistical Applications in Genetics and Molecular Biology (2004)
We present a series of statistical solutions to challenges that commonly arise in the production...
Endosymbiosis
Sequestration, performance, and functional control of cryptophyte plastids in the ciliate Myrionecta rubra (Ciliophora), Journal of Phycology (2006)
Myrionecta rubra (Lohmann 1908, Jankowski 1976) is a photosynthetic ciliate with a global distribution in...
Heterotachy processes in rhodophyte-derived secondhand plastid genes: implications for addressing the origin and evolution of dinoflagellate plastids, Molecular Biology and Evolution (2006)
Serial transfer of plastids from one eukaryotic host to another is the key process involved...
Phylogenetic analyses indicate that the 19'Hexanoyloxy-fucoxanthin-containing dinoflagellates have tertiary plastids of haptophyte origin, Molecular Biology and Evolution (2000)
The three anomalously pigmented dinoflagellates Gymnodinium galatheanum, Gyrodinium aureolum, and Gymnodinium breve have plastids possessing...
Genetically Modified Organisms (GMOs)
Characterization of unknown genetic modifications using high throughput sequencing and computational subtraction, BMC Biotechnology (2009)
Background
When generating a genetically modified organism (GMO), the primary goal is to give...
Non-prejudiced Detection and Characterization of Genetic Modifications, Food Analytical Methods (2009)
The application of gene technology is becoming widespread much thanks to the rapid increase in...
A statistical approach for evaluation of PCR results to improve the practical limit of quantification (LOQ) of GMO analyses (SIMQUANT), European Food Research and Technology (2008)
The predominant approach for quantification of genetically modified organisms (GMO) is the application of quantitative...
Microarray-based method for detection of unknown genetic modifications, BMC Biotechnology (2007)
Background
Due to the increased use of genetic modifications in crop improvement, there is...
Equal performance of TaqMan, MGB, molecular beacon, and SYBR green-based detection assays in detection and quantification of Roundup Ready soybean, Journal of Agricultural and Food Chemistry (2006)
We have tested and compared the performance of 12 different assays representing four different real-time...
Harmful Algae
Identical ribosomal DNA sequence data from Pfiesteria piscicida (Dinophyceae) isolates with different toxicity phenotypes, Environmental Research (2003)
Complete small subunit ribosomal RNA, internal transcribed spacer 1 and 2, 5.8S, and partial large...
Kryptoperidinium foliaceum blooms in South Carolina: a multi–analytical approach to identification, Harmful Algae (2002)
Observations following the discovery of Kryptoperidinium foliaceum blooms in South Carolina (SC), USA, suggest that...
No difference found in ribosomal DNA sequences from physiologically diverse clones of Karenia brevis (Dinophyceae) from the Gulf of Mexico, Journal of Plankton Research (2002)
Maximum growth rate and toxin content were significantly different among five strains of Karenia brevis...
Discovery of the toxic dinoflagellate Pfiesteria in northern European waters, Proceedings of the Royal Society of London (2002)
Several dinoflagellate strains of the genus Pfiesteria were isolated by culturing techniques from sediment samples...
Heteroduplex mobility assay-guided sequence discovery: elucidation of the small subunit (18S) rDNA sequences of Pfiesteria piscicida and related dinoflagellates from complex algal culture and environmental sample DNA pools, Proceedings of the National Academy of Sciences of the United States of America (2000)
The newly described heterotrophic estuarine dinoflagellate Pfiesteria piscicida has been linked with fish kills in...
Molecular Detection
A quantitative TaqMan MGB real-time polymerase chain reaction based assay for detection of the causative agent of crayfish plague Aphanomyces astaci, Veterinary Microbiology (2009)
Here we present the development and first validation of a TaqMan minor groove binder (MGB)...
Development of real–time PCR assays for the detection of Chattonella species in culture and environmental samples, Harmful Algae 2002 (2004)
Raphidophytes have been associated with fish kill events in Japanese, European and U.S. coastal waters,...
Use of molecular probes to assess geographic distribution of Pfiesteria species, Environmental Health Perspectives (2001)
We have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp....
Genetic polymorphism in Gymnodinium galatheanum chloroplast DNA sequences and development of a molecular detection assay, Molecular Ecology (2001)
Nuclear and chloroplast-encoded small subunit ribosomal DNA sequences were obtained from several strains of the...
Real–time PCR monitoring of estuarine water samples for Pfiesteria piscicida, a dinoflagellate associated with fish kills and human illness, Rapid Cycle Real–Time PCR: Methods and Applications (2001)
No abstract is available for this book chapter.
Molecular Methods
Genomic representations using concatenates of type IIB restriction endonuclease digestion fragments, Nucleic Acids Research (2004)
We have developed a method for genomic representation using Type IIB restriction endonucleases. Representation by...
Taxonomy/Systematics
Francisella philomiragia subsp. noatunensis subsp. nov., isolated from farmed Atlantic cod (Gadus morhua L.), International Journal of Systematic and Evolutionary Microbiology (2007)
Seven bacterial isolates from farmed Atlantic cod displaying chronic granulomatous disease were characterized by phenotypic...
Phenotypically different microalgal morphospecies with identical ribosomal RNA: a case of rapid adaptive evolution?, Microbial Ecology (2007)
The agents driving the divergence and speciation of freeliving microbial populations are still largely unknown....
Raphidophyceae [Chadefaud ex Silva] systematics and rapid identification: sequence analyses and real–time PCR assays, Journal of Phycology (2006)
Species within the class Raphidophyceae were associated with fish kill events in Japanese, European, Canadian,...
A group of dinoflagellates similar to Pfiesteria as defined by morphology and genetic analysis, Harmful Algae (2006)
Eight novel pfiesteria-similar dinoflagellates are described that form a genetically well-defined group with distinct morphological...
Highly divergent SSU rRNA genes found in the marine ciliates Myrionecta rubra and Mesodinium pulex, Protist (2004)
Myrionecta rubra and Mesodinium pulex are among the most commonly encountered planktonic ciliates in coastal...